Mass Spectrometry Peptidomics Data for Crustacean Hemolymph Peptides

Method 3, combining anticoagulants and protease inhibitors with a 10 kDa filtration step, achieved the highest peptide identification rates in a study of American lobster and blue crab hemolymph. Tina C. Dang published this dataset on figshare in 2026, detailing workflows for recovering endogenous peptides. The data includes identifications of antimicrobial peptides and neuropeptides, including novel putative sequences.

Use Cases

• Benchmarking peptide extraction workflows based on the five evaluated methods described.
• Identifying novel antimicrobial peptides based on distinct amino acid motifs reported for Callinectes sapidus and Homarus americanus.
• Analyzing neuropeptide recovery performance across different sample preparation techniques.
• Studying the impact of reduction and alkylation steps on peptide identification rates as demonstrated in the description.

Strengths

• Dataset size is 73.5 KB, indicating a focused and manageable scope for analysis.
• The description provides specific methodological comparisons, such as an approximately 40% decrease in AMP identifications with Method 4.
• Reports concrete findings like six novel putative neuropeptides in C. sapidus and nine and eleven in silico-predicted novel AMPs.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Data may reflect methodological bias inherent to the specific extraction workflows tested.

Provenance

Source

figshare

Collection Method

Mass spectrometry-based peptidomic analysis of hemolymph from Homarus americanus and Callinectes sapidus, evaluating five extraction workflows.

Freshness

Last updated 2026-05-05 06:14:28; freshness should be verified.