Metabolite Profiles from Sorted Drosophila Testes Cells

Six replicates of mass spectrometry data capture metabolites from sorted somatic cyst and germ cells of Drosophila testes. Analysis used hydrophilic interaction liquid chromatography and an Orbitrap QExactive in polarity switching mode, with metabolite identity confirmed by matching to authentic standards. The dataset comprises .mzXML files separated for positively-charged and negatively-charged metabolites.

Use Cases

• Comparative metabolomic analysis between somatic cyst cells and germ cells.
• Identification of cell-type-specific metabolites based on accurate mass and retention time.
• Method validation for metabolite extraction and LC-MS protocols from sorted Drosophila tissues.

Strengths

• Includes six biological replicates for each cell type, supporting statistical analysis.
• Metabolite identity was confirmed by running authentic standards alongside samples.
• Data is publicly available under a permissive CC0 1.0 license.

Limitations

• Column names and row counts are not provided, limiting immediate understanding of data structure.
• The dataset consists of raw .mzXML files, requiring specialized bioinformatics tools for processing and interpretation.

Provenance

Source

Polyomics Facility at the University of Glasgow

Collection Method

Cells were dissociated from dissected Drosophila testes, sorted via FACS (BD FACSAria Fusion), and metabolites were extracted using chloroform/methanol/water. Analysis used a Dionex UltiMate 3000 RSLC system with a ZIC-pHILIC column.

Time Range

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Freshness

2026-03-24 09:59:28

Geography

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