Differential Scanning Calorimetry Measurements for Biological Substances

Hans‐Jürgen Hinz authored a paper describing the measurement and analysis of biological substances using Differential Scanning Calorimetry (DSC). The description details procedures for DSC measurement, calibration, and performance testing, including an inter-laboratory study on hen egg white lysozyme. The dataset likely contains thermodynamic transition data, such as temperature and enthalpy values, for proteins, nucleic acids, and lipid assemblies.

Use Cases

• Modeling protein unfolding thermodynamics based on transition temperature and enthalpy data.
• Calibrating Differential Scanning Calorimetry instruments using the described standard procedures.
• Comparing thermodynamic transition models (e.g., two-state, three-state) on heat capacity vs. temperature plots.
• Assessing inter-laboratory measurement consistency using the provided lysozyme benchmark data.

Strengths

• Description includes specific benchmark results: average unfolding temperature of 331.2 K and enthalpy of 405 kJ mol–1 for lysozyme.
• Provides detailed methodological recommendations for DSC measurement, calibration, and data analysis.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Last update date is unknown; freshness unverified.

Provenance

Source

Hans‐Jürgen Hinz

Collection Method

Likely contains experimental data from Differential Scanning Calorimetry measurements, including an inter-laboratory study.

Geography

Data from six different DSC laboratories worldwide are referenced.