COI Metabarcoding: Illumina NextSeq Library Metadata for Ecological Analysis

Metadata from an Illumina NextSeq2000 metabarcoding library using the COI primer pair BF3 and BR2. The CSV file includes both field and control samples, formatted for ecological analyses using R packages like phyloseq and vegan. Author Alejandro Maeda Obregon published the dataset on figshare under a CC-BY-4.0 license, with a last update timestamp of 2026-04-28 12:43:04.

Use Cases

• Assess biodiversity in field samples based on COI gene sequences.
• Compare species communities between field and control samples.
• Perform ecological analyses like alpha/beta diversity using the R package vegan.
• Manage and analyze sequence data within the R package phyloseq.

Strengths

• Includes both field and control samples, which can aid in quality control.
• Formatted for direct use with established ecological analysis R packages (phyloseq, vegan).
• Published under a permissive CC-BY-4.0 license, allowing reuse and modification.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare

Collection Method

Illumina NextSeq2000 sequencing with COI primer pair (BF3 and BR2).

Freshness

Last updated 2026-04-28 12:43:04; freshness should be verified.