AU_AADC research data details the expression and preliminary characterization of metalloproteins by the Antarctic bacterium Shewanella gelidimarina. The dataset includes results from protein purification, N-terminal sequencing, and enzyme activity assays like the Greiss reaction for nitrite production. Data was last updated by the Australian Antarctic Data Centre in July 2003.
Use Cases
- Analyze N-terminal sequence homology (e.g., 'A D P L T V Y G K L N V T A Q S N D V N') to identify putative outer membrane porins against known databases.
- Correlate growth conditions (e.g., 4°C, iron-limited, nitrate-supplemented media) with enzyme activity measurements from Greiss assay results.
- Study protein properties like molecular weight (42 kDa from denaturing PAGE) expressed under specific phenotyped bacterial cultures.
Strengths
- Data includes specific molecular measurements like a 20-residue N-terminal sequence and a 42 kDa protein mass.
- Research context links protein expression to defined environmental conditions (Antarctic, 4°C, specific media supplements).
Limitations
- Dataset size and sample count are unknown, suggesting a small-scale experimental study.
- Data is temporally stale, with no updates recorded since July 2003.
Provenance
- Source
- Australian Antarctic Data Centre (AU_AADC), accessed via NASA Earthdata.
- Collection Method
- Laboratory analysis of cultured Shewanella gelidimarina, including protein purification, PAGE, N-terminal sequencing, and enzyme activity assays.
- Time Range
- Collection date unknown; data publication circa 2003.
- Freshness
- 2003-07-31
- Geography
- Antarctic; bacteria sourced from Antarctic sea-ice environments.