ISS-MO: Microbial Diversity on International Space Station Surfaces
Updated 4mo ago
5filesBIN
Available on 1 platform
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Description
Two consecutive sampling sessions three months apart collected environmental microbiome data from eight locations on the International Space Station. The dataset, created by the National Aeronautics and Space Administration, includes results from 16S and ITS iTag Illumina sequencing, ATP assays, and PMA-qPCR analysis to measure viable microbial diversity. Samples were retrieved via Soyuz TMA-14M or SpaceX Dragon capsule and analyzed upon return to Earth.
Use Cases
Analyzing spatial distribution of viable microbes based on sampling from eight different ISS locations.
Studying temporal changes in microbial populations based on two sampling sessions three months apart.
Comparing cultivable versus non-cultivable microbial diversity using data from polyester wipes and contact slides.
Investigating the impact of environmental factors like microgravity and radiation on microbiome composition.
Strengths
Data includes viable microbial diversity distinguished using PMA assay before DNA extraction.
Samples cover eight different surface locations on the ISS, allowing for spatial analysis.
Two consecutive sampling sessions three months apart provide temporal comparison points.
Uses multiple analysis methods: 16S/ITS sequencing, ATP assay, PMA-qPCR, and cultivation on different agar types.
Limitations
Row count and dataset size are unknown, which may limit suitability assessment.
Column-level documentation is absent; field semantics must be inferred after download.
File format is listed as BIN, which may require specific tools for access and processing.
Provenance
Source
National Aeronautics and Space Administration
Collection Method
Samples collected with polyester wipes and contact slides from ISS surfaces, analyzed with molecular techniques including sequencing, ATP assay, and qPCR.
Time Range
Two sampling sessions conducted three months apart.
Freshness
Last updated 2026-03-13 19:16:21.129751; freshness should be verified.
Geography
International Space Station (ISS).
License is listed as 'other-license-specified'; specific terms must be reviewed before use.