MicroED Datasets of Hemin and Biotin Microcrystals from Ceta Camera

MicroED datasets of hemin and biotin microcrystals collected using a Talos Arctica microscope and Ceta CMOS camera. ~14 biotin datasets merge at ~0.85 Å resolution, and ~12 hemin datasets merge at ~0.9 Å resolution, with detailed collection parameters provided. The data was contributed by Haruaki Yanagisawa from The University of Tokyo and is stored in compressed EMD (HDF5) format with metadata in JSON.

Use Cases

• Developing or testing MicroED data processing pipelines based on the provided EMD file format and metadata structure.
• Benchmarking software like DIALS for electron diffraction analysis using the described crystal parameters and rotation steps.
• Training computer vision models for diffraction pattern recognition using the ~40 image frames per dataset with constant rotation speed.
• Studying the structural properties of hemin and biotin based on the provided space group and unit cell dimensions.

Strengths

• Provides high-resolution structural data, with biotin datasets merged at ~0.85 Å and hemin at ~0.9 Å resolution.
• Includes detailed experimental parameters such as camera length (793.52 mm), rotation step (~1.52°/frame), and electron dose rates.
• Data is stored in a structured EMD (HDF5) format with machine parameters and stage tilt angles stored as JSON metadata.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for large-scale ML tasks.
• Last update date is unknown; freshness unverified.

Provenance

Source

Haruaki Yanagisawa, The University of Tokyo

Collection Method

Collected using Talos Arctica (200 kV) microscope with Ceta CMOS camera, controlled by SerialEM and Velox software.