Microgravity Proteomics in Cardiomyocytes Using Label-Free and Dynamic SILAC

Mass-spectrometry-based proteomics reveals molecular changes in primary cardiomyocytes under microgravity stress. The study employs both label-free quantification and dynamic stable-isotope labeling by amino acids in cell culture (Dynamic SILAC) techniques. Data was produced by the National Aeronautics and Space Administration and last updated in March 2026.

Use Cases

• Identify proteins involved in endoplasmic reticulum stress based on relative quantitation data.
• Model mitochondrial protection pathways using dynamic protein content changes from the SILAC component.
• Compare cellular stress response mechanisms between microgravity and normal gravity conditions.
• Train ML models to classify proteomic signatures of specific cellular stressors.

Strengths

• Proteomics analysis allows for the relative quantitation of a large number of proteins concurrently.
• Combines two techniques: label-free quantification and dynamic stable-isotope labeling (Dynamic SILAC).
• Data originates from the authoritative source National Aeronautics and Space Administration.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data is provided in a BIN file format, which may require specialized tools for access.

Provenance

Source

National Aeronautics and Space Administration

Collection Method

Mass-spectrometry based proteomics on primary cardiomyocytes, using label-free quantification and Dynamic SILAC.

Freshness

Last updated 2026-03-13 19:27:48.057320; freshness should be verified.