Mitochondrial Genome Sequencing Data from Chinese Xibe Population for Forensic Analysis

A study employed short overlapping amplicons and the MiSeq FGx system for whole mitochondrial genome sequencing. The dataset includes results from 103 Chinese Xibe individuals, identifying 103 unique haplotypes and 3,962 variants. The research was conducted by Xin Yang of Qingdao University and contributed to the EMPOP database.

Use Cases

• Forensic mixture deconvolution based on analysis of minor contributors in DNA mixtures with ratios from 19:1 to 1:19
• Haplogroup assignment and population studies based on 103 unique mitogenome haplotypes from the Chinese Xibe population
• Method validation for degraded DNA analysis based on variant detection from samples with fragments ≥200 base pairs
• Assessing sequencing depth and accuracy based on an averaged depth of coverage exceeding 1800x at each position

Strengths

• High sequencing depth with an averaged depth of coverage exceeding 1800x at each position
• Includes data from 103 Chinese Xibe individuals, resulting in 103 unique mitogenome haplotypes assigned to 79 haplogroups
• Demonstrates sensitivity with full variants obtained from input DNA as low as 50 picograms
• Reports 3,962 variants observed at 613 nucleotide positions, providing a detailed variant catalog

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• Row count is unknown, which may limit suitability assessment
• Last update date is unknown; freshness unverified

Provenance

Source

Qingdao University, contributed to EMPOP (European DNA Profiling Group's Mitochondrial DNA Population Database)

Collection Method

Sequencing performed using short overlapping amplicons, library preparation with Nextera XT kit, and analysis with mtDNA MSR Plug-in and Variant Analyzer on the MiSeq FGx Forensic Genomics System.

Geography

Data is from the Chinese Xibe population, with principal component analysis showing clustering with East and Southeast Asian groups.