Molecular Detection of Anaplasma Species in Dogs from Central-Western Colombia, 2008-2009

Colombian dog blood samples from Bogota, Villavicencio, and Bucaramanga were analyzed between December 2008 and April 2009. The work by Giovanni Vargas-Hernández involved molecular detection of Anaplasma species in 91 dogs using 16S rRNA nPCR, sequencing, and phylogenetic analysis. It reports a low frequency of detection, including A. platys and an Anaplasma sp. related to A. phagocytophilum.

Use Cases

• Studying the prevalence of Anaplasma platys in dogs based on PCR and sequencing results mentioned in the description
• Investigating the circulation of Anaplasma species phylogenetically related to A. phagocytophilum based on the molecular findings
• Analyzing regional patterns of tick-borne pathogens in Colombia based on the sample collection from three cities
• Benchmarking molecular detection techniques (nPCR, real-time qPCR) for Anaplasmataceae agents as described in the study

Strengths

• Sample size of 91 dogs provides a basis for prevalence estimation.
• Specific time range (December 2008 to April 2009) and geographic locations (three Colombian cities) are documented.
• Employs multiple molecular techniques (16S rRNA nPCR, sequencing, phylogenetic analysis, specific qPCR) for detection and identification.

Limitations

• Row count and column-level documentation are absent; field semantics must be inferred after download.
• Data may reflect geographic and temporal bias inherent to a single, small-scale study from paperswithcode.
• Last update date is unknown; freshness unverified.

Provenance

Source

Giovanni Vargas-Hernández

Collection Method

Blood samples drawn from dogs, analyzed using 16S rRNA-Anaplasma spp. nPCR, sequencing, phylogenetic analysis, and blood smear preparation.

Time Range

December 2008 to April 2009

Geography

Central-western region of Colombia (cities of Bogota, Villavicencio, and Bucaramanga)