Lake Trout Transcriptomic Responses to 6PPD-Quinone Toxicity

Transcriptomic analysis reveals concentration-dependent dysregulation of inflammation, oxidative stress, and developmental pathways in lake trout alevins exposed to the tire rubber contaminant 6PPD-quinone. The dataset contains benchmark concentration modeling results, including a transcriptomic BMC10th of 0.211 μg/L. Researcher Junyi Lin published this toxicological study on figshare in April 2026.

Use Cases

• Identify transcriptomic points of departure (tPODs) by analyzing benchmark concentration (BMC) values for gene expression changes.
• Model concentration-response relationships using graded exposure data from 0.22 to 7.6 μg/L 6PPD-quinone.
• Investigate molecular toxicity pathways by examining dysregulated gene sets related to inflammation, vascular integrity, and ferroptosis.
• Compare transcriptomic BMC10th values (0.211 μg/L) with traditional apical toxicity thresholds (0.161-0.252 μg/L) for risk assessment.

Strengths

• Benchmark concentration modeling provides quantitative toxicity thresholds (e.g., BMC10th of 0.211 μg/L).
• Exposure concentrations are precisely defined across a graded range (0.22–7.6 μg/L).
• Analysis covers specific molecular pathways including inflammation, oxidative stress, and development.

Limitations

• Dataset size is 6.2 MB, indicating a limited scope likely focused on summary results rather than raw sequencing data.
• Sample size (number of biological replicates) for the transcriptomic analysis is not specified.
• Findings are specific to early-life stage lake trout (Salvelinus namaycush), limiting generalizability to other species or life stages.

Provenance

Source

figshare, authored by Junyi Lin.

Collection Method

Whole-transcriptome analysis of lake trout alevins exposed to 6PPD-quinone for 96 hours, followed by benchmark concentration modeling.

Time Range

Experimental study, temporal coverage not explicitly stated.

Freshness

Last updated in April 2026.

Geography

Study focuses on the species lake trout (Salvelinus namaycush), significant in North America; specific sample origin not stated.