MouseLight Neuron AA1507: 3D Reconstructions of Over 300 Mouse Brain Neurons

More than 300 fully-traced neurons from the mouse brain are available for download and 3D browsing, representing a tenfold increase over previous efforts. The data was created by Janelia scientists using iterative two-photon microscopy on 200-micron brain slices, with each brain scan generating about 50 terabytes of raw data. The selectively-labeled neurons, some with fibers up to 45 centimeters long, can be explored via the NeuronBrowser application.

Use Cases

• Analyze neural morphology and branching patterns based on the 3D neuron reconstructions.
• Study long-range brain connectivity based on the tracing of individual neurons across hemispheres.
• Validate or train computational models of neural networks based on the detailed wiring map.
• Visualize and explore the spatial organization of neural circuits in 3D.

Strengths

• Contains reconstructions of more than 300 neurons, a tenfold increase over previous datasets.
• Neurons are traced in high detail, with some fibers mapped up to 45 centimeters long.
• Data is available in 3D formats (SWC, JSON) for interactive exploration and analysis.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is small (382.1 KB), representing a limited sample of the 70 million neurons in a mouse brain.

Provenance

Source

Janelia Research Campus

Collection Method

Selectively-labeled neurons were mapped in an iterative process with two-photon microscopy on 200-micron brain slices.

Freshness

Last updated 2026-05-13 19:33:31; freshness should be verified.