Candida Auris Echinocandin Resistance Mechanisms with Biofilm and Cell Wall Data

A 44.1 MB research dataset investigates echinocandin resistance in the fungal pathogen Candida auris. The study by Xi Yang, uploaded in April 2026, includes experimental data on genetic mutations, biofilm formation assays, and cell wall component analysis. It provides a multifaceted framework for understanding antifungal resistance.

Use Cases

• Correlate the FKS1 S639F mutation with echinocandin susceptibility levels measured by E-test.
• Analyze biofilm formation data from crystal violet and XTT assays under anidulafungin treatment stress.
• Model cell wall remodeling by quantifying changes in chitin, mannan, and β-glucan levels from fluorescence assays.
• Investigate gene expression relationships by linking RT-qPCR data for FKS1, CHS1, and PMR1 with pathway activation.
• Evaluate the additive effect of calcineurin inhibitors with anidulafungin using results from disk diffusion and checkerboard assays.

Strengths

• Data includes multiple experimental modalities: genetic sequencing, biochemical assays, and gene expression analysis.
• Research focuses on a specific, clinically critical isolate (C. auris 01) with a defined resistance mutation.
• Dataset is openly licensed under CC-BY-4.0, facilitating reuse and analysis.

Limitations

• Dataset scale is limited at 44.1 MB, primarily containing document and image files rather than large, structured data tables.
• Sample size is focused on a single primary clinical isolate, limiting generalizability across strains.
• Data is presented in document and image formats (TIF, DOCX), requiring manual extraction for computational analysis.

Provenance

Source

figshare, authored by Xi Yang.

Collection Method

Laboratory study using E-test susceptibility, Sanger sequencing, crystal violet/XTT biofilm assays, quantitative fluorescence, RT-qPCR, and disk diffusion/checkerboard assays.

Time Range

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Freshness

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Geography

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