Naive B Cells, Antibody Secreting Cells, Plasma Cells: Mouse Immune Cell RNA-Seq Data

Raw Illumina MiSeq data in zipped FASTQ format includes demultiplexed samples from naive B cells, antibody secreting cells, and plasma cells obtained from a single Balb/c mouse via sorting. Every sample consists of forward and reverse reads with 2x250 bp length. The dataset was contributed by Victor Greiff from ETH Zurich.

Use Cases

• Analyzing B cell receptor repertoires based on the paired-end RNA sequencing reads.
• Comparing gene expression profiles between naive B cells, antibody secreting cells, and plasma cells from the same mouse.
• Developing sequence alignment or assembly pipelines for immune cell data based on the 2x250 bp FASTQ format.

Strengths

• Data includes matched samples from three distinct B cell lineages (naive, antibody secreting, plasma) from a single mouse, allowing for controlled comparisons.
• Sequencing reads are paired-end with a defined length of 250 base pairs per read.

Limitations

• Row count and total data size are unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Last update date is unknown; freshness unverified.

Provenance

Source

Victor Greiff, ETH Zurich, via paperswithcode.

Collection Method

Cells were obtained from a Balb/c mouse via sorting and sequenced on an Illumina MiSeq platform.