New Reversible Covalent Warheads: Proteome-Wide Map of Ligandable N-Terminal Cysteines

A proteomics dataset mapping ligandable native N-terminal cysteine (NCys) residues across the human proteome. The data was generated by Jixian Zhang using a library of electrophilic probes, including cyanobenzothiazole (CBT), and published on figshare in April 2026. It identifies modifiable NCys sites in proteins such as GFPT1 and CSTB.

Use Cases

• Identifying novel drug targets based on ligandable N-terminal cysteine sites mentioned in the description
• Developing reversible covalent degraders based on the cyanobenzothiazole (CBT) platform described
• Benchmarking degradation efficiency using reported metrics like DC50 and Dmax
• Studying ternary complex formation and ubiquitination mechanisms for proteins like DCAF16

Strengths

• Provides the first proteome-wide map of ligandable NCys residues, a novel resource
• Demonstrates potent degradation metrics (e.g., DC50 = 16.7 nM, Dmax = 98%) for a BRD4-targeting degrader
• Shows platform efficacy against challenging targets like EGFR L858R/T790M/C797S and HER2 without hook effects

Limitations

• Row count is unknown, which may limit suitability assessment
• Column-level documentation is absent; field semantics must be inferred after download
• Dataset is very small (2.1 KB), indicating limited scope or summary-level data

Provenance

Source

figshare

Collection Method

Constructed using a library of electrophilic probes to systematically map ligandable native NCys residues.

Time Range

Publication date is 2026, but experimental time range is unspecified.

Freshness

Last updated 2026-04-28 03:05:36; freshness should be verified

Geography

Focus is on the human proteome; geographic origin of samples is unspecified.