Opsonization-Enhanced Dual Proteomic Analysis of Burkholderia cenocepacia Infections

Michael G. Bacus published a proteomics dataset on figshare in 2026. The data likely contains protein expression changes in both host macrophages and internalized Burkholderia cenocepacia bacteria at 3 and 24 hours post-infection. The dataset is 15.5 KB and is provided in an XLSX file format.

Use Cases

• Analyzing host macrophage proteomic response to bacterial infection based on the description of proinflammatory signaling and activation markers.
• Studying bacterial protein expression changes during intracellular replication based on the dual proteomic analysis framework.
• Investigating the functional role of the Type 6 Secretion System (T6SS) and its effector TecA based on the described experimental assessment.
• Developing methods for improving proteomic analysis of low-infectivity pathogens based on the described opsonization technique.

Strengths

• Dataset is openly available under a CC-BY-NC-4.0 license.
• The experimental method is described, involving a dual proteomic analysis at two time points (3h and 24h).
• The dataset is focused on a specific, well-defined biological model (B. cenocepacia infection of THP-1 macrophages).

Limitations

• The dataset is very small at 15.5 KB, indicating limited scope.
• Row count and column-level documentation are unknown, requiring manual inspection after download.
• The description lacks specific details on the number of biological replicates or quantified protein features.

Provenance

Source

figshare

Collection Method

Proteomic analysis of THP-1 macrophage cells infected with Burkholderia cenocepacia, with and without antibody-mediated opsonization.

Freshness

Last updated 2026-05-28 23:45:15.