Opsonization-Enhanced Dual Proteomic Analysis of Burkholderia cenocepacia Infections

39.1 MB of proteomic data from a study investigating host-pathogen interactions during Burkholderia cenocepacia infection of macrophages. The dataset, authored by Michael G. Bacus and last updated in May 2026, was generated using an antibody-mediated opsonization technique to improve infection uniformity for dual proteomic analysis. It assesses the impact of the bacterial type 6 secretion system and its effector TecA at 3 and 24 hours post-infection.

Use Cases

• Analyzing host proteomic changes during bacterial infection based on the described proinflammatory signaling and macrophage activation markers.
• Studying bacterial proteomic adaptation during intracellular replication based on the dual proteomic framework.
• Investigating the role of the type 6 secretion system (T6SS) in infection based on the comparative analysis of T6SS and TecA mutant strains.
• Benchmarking methods for improving proteomic analysis of low-infectivity pathogens based on the opsonization technique described.

Strengths

• Dataset size is 39.1 MB, indicating a substantive amount of proteomic measurements.
• The experimental framework includes dual proteomic analysis of both host and pathogen within a single system.
• Analysis includes specific time points (3 and 24 hours post-infection) for temporal dynamics.

Limitations

• Row count is unknown, which may limit suitability assessment for large-scale statistical modeling.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is focused on a specific in vitro model (THP-1 cells), which may limit generalizability.

Provenance

Source

figshare

Collection Method

Proteomic data generated from in vitro infection models of THP-1 macrophages with Burkholderia cenocepacia, using antibody-mediated opsonization to enhance infectivity.

Freshness

Last updated 2026-05-28 23:45:18.