Opsonization-Enhanced Dual Proteomic Analysis of Burkholderia cenocepacia Infections

A 19.1 MB proteomics dataset from figshare, last updated on 2026-05-28. The data was generated by Michael G. Bacus to study host and bacterial protein changes during macrophage infection by the opportunistic pathogen Burkholderia cenocepacia. The work demonstrates a method using antibody-mediated opsonization to improve infection efficiency for dual proteomic analysis.

Use Cases

• Analyzing host proteomic changes in response to bacterial infection based on the description of proinflammatory signaling and macrophage activation markers.
• Studying bacterial proteomic adaptation during intracellular replication based on the described dual proteomic framework.
• Evaluating the impact of specific bacterial virulence factors, such as the type 6 secretion system (T6SS) and the effector TecA, on host response based on the experimental assessment mentioned.
• Benchmarking methods to improve proteomic analysis of low-infectivity pathogens based on the described opsonization technique.

Strengths

• Dataset size is 19.1 MB, indicating a contained but substantive proteomics dataset.
• The description provides a clear experimental framework, including the use of opsonization and analysis at 3 and 24 hours post-infection.
• Data is shared under a CC-BY-NC-4.0 license, allowing for non-commercial reuse with attribution.

Limitations

• Row count is unknown, which may limit suitability assessment for specific analytical needs.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset appears to be a single, static snapshot with no stated update frequency.

Provenance

Source

figshare

Collection Method

Likely generated via mass spectrometry proteomic analysis of infected THP-1 macrophage cells, as described.

Time Range

Experimental time points include 3 and 24 hours post-infection.

Freshness

Last updated 2026-05-28 23:45:12; freshness should be verified.