OpticalFlow3D: Myosin II 3D Timelapse Images and Analysis Scripts

10.2 GB of 3D timelapse images and Jupyter notebooks for analyzing myosin II protein dynamics. The dataset, authored by Rachel Lee and shared under a CC-BY-4.0 license, includes a main '3D_timelapse' folder and subsets used for generating figures. Methods are detailed in an associated 2026 bioRxiv publication.

Use Cases

• Applying optical flow algorithms to 3D timelapse images of myosin II based on the provided Jupyter notebooks.
• Reproducing scientific figures from the associated publication using the provided scripts and data subsets.
• Developing new image analysis pipelines for protein dynamics using the 3D datasets as a benchmark.

Strengths

• Dataset size is 10.2 GB, indicating a substantial volume of image data.
• Includes executable Jupyter notebooks ('run_flow_myosin.ipynb') for direct analysis and figure generation.
• Linked to a specific, peer-reviewed pre-print publication detailing the imaging and analysis methods.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for certain ML tasks.
• The description metadata is limited to file organization and basic usage instructions.

Provenance

Source

Rachel Lee via figshare.

Collection Method

Methods for imaging and analysis are described in the associated bioRxiv publication.

Freshness

Last updated 2026-05-26 19:29:16; freshness should be verified.