Biotin-Drug Acid Dissociation Data for BEAD ADA Assay Optimization

Acid-induced dissociation of biotin-labeled drugs from streptavidin-coated beads was characterized using a modified ADA bridging assay and Biolayer Interferometry. The dataset, created by Lili Guo and shared under CC-BY-4.0, contains JPG files totaling 301.6 KB and was last updated in May 2026. Dissociation magnitude was found to depend on acid type and pH, with acetic acid at pH 2.65, lactic acid at pH 2.23, and glycine at pH 3.00 producing the greatest dissociation.

Use Cases

• Optimizing acid selection and pH conditions for biotin-drug dissociation based on the described experimental findings.
• Improving drug tolerance in ADA workflows based on the proposed reagent tuning and optimization framework.
• Benchmarking dissociation rates under different acidic conditions based on the described BLI and bridging assay results.
• Developing systematic assay optimization workflows focusing on reagent concentrations and bead amounts as described.

Strengths

• Provides specific experimental results on dissociation performance for acids like acetic, lactic, and glycine at defined pH levels.
• Includes a proposed fit-for-purpose optimization framework for improving assay efficiency.
• Shared under a permissive CC-BY-4.0 license, allowing for reuse and modification.

Limitations

• Dataset is very small (301.6 KB), indicating limited scope.
• Row count and column-level documentation are absent; data structure must be inferred from the JPG files.
• The description focuses on methodology and findings; the actual data files' content and format require inspection.

Provenance

Source

figshare

Collection Method

Data likely generated from modified ADA bridging assays and Biolayer Interferometry (BLI) analysis as described.

Time Range

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Freshness

Last updated 2026-05-19 07:42:51; freshness should be verified.

Geography

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