Yarrowia Lipolytica Squalene Production Metabolic Profiles

A research dataset from Beijing University of Chemical Technology details metabolic engineering experiments for squalene production. The data likely contains measurements from engineered Yarrowia lipolytica strains, including squalene titers, metabolic byproduct levels, and fermentation conditions. It was authored by Huan Liu and shared via the paperswithcode platform.

Use Cases

• Benchmarking squalene production yields based on reported titers of up to 502.7 mg/L.
• Analyzing the impact of pathway bottlenecks based on the identification of HMG-CoA reductase as a key target.
• Modeling cofactor recycling strategies based on the described NADPH regeneration from the mannitol cycle.
• Optimizing fermentation conditions based on described C/N ratio, pH control, and acetyl-CoA flux mitigation.

Strengths

• Reports specific quantitative outcomes, including a 28-fold increase in squalene titer.
• Describes systematic engineering steps, including bottleneck identification and strain optimization.
• Includes data from multiple minimal media conditions (glucose and acetate).

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data may reflect the specific experimental bias inherent to a single research paper.

Provenance

Source

Beijing University of Chemical Technology

Collection Method

Experimental data from metabolic engineering and fermentation studies.