Toxoplasma Gondii Electroporation Performance and Buffer Comparison Data

A 38.3 KB dataset supporting a study on optimizing genetic manipulation protocols for the intracellular parasite Toxoplasma gondii. Pratik Narain Srivastava published the data in April 2026, comparing a modified Opti-MEM I buffer against a standard cytomix buffer. It includes a novel scoring method (etScore) for quantifying electroporation performance.

Use Cases

• Compare transfection efficiency metrics between the modified Opti-MEM I and cytomix buffers.
• Apply the etScore method to quantify electroporation performance combining transgene expression and cell viability data.
• Conduct systematic risk assessments for genetic manipulation protocols using the provided framework.
• Benchmark new electroporation buffer formulations against the performance data in this study.

Strengths

• Includes a novel, reproducible scoring method (etScore) for electroporation quantification.
• Data supports a direct performance comparison between two specific electroporation buffers.
• Released under a permissive CC-BY-4.0 license for broad reuse.

Limitations

• Very small dataset at 38.3 KB, indicating limited raw experimental measurements.
• Specific row counts, column names, and sample data are not provided for detailed analysis.
• Data scope is narrowly focused on a single parasite species and a specific genetic technique.

Provenance

Source

Figshare repository, authored by Pratik Narain Srivastava.

Collection Method

Experimental data from electroporation trials on Toxoplasma gondii, likely involving transfection efficiency and cell viability measurements.

Time Range

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Freshness

Last updated in April 2026, indicating recent experimental work.

Geography

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