Experimental data characterizing the efficiency and accuracy of in vivo linear DNA assembly methods, specifically RecET and λ Red recombination systems. The dataset was authored by James A. Sawitzke and last updated on April 3, 2026. It is a small 5.5 KB file in XLS format.
Use Cases
- Benchmarking assembly efficiency based on comparisons between RecET and λ Red systems.
- Evaluating error rates in DNA fragment assembly based on the description of accurate joining.
- Assessing the impact of Exonuclease I deletion on assembly performance for multi-fragment constructs.
Strengths
- Data is licensed under CC-BY-4.0, allowing for open reuse and modification.
- Dataset includes comparisons to a commercial in vitro method (NEBuilder® HiFi DNA Assembly).
- Last update is recent (2026-04-03).
Limitations
- Dataset is very small (5.5 KB), indicating limited scope.
- Row count and column-level documentation are absent; field semantics must be inferred after download.
Provenance
- Source
- James A. Sawitzke via figshare
- Collection Method
- Likely contains experimental results from laboratory characterization of DNA assembly parameters.
- Time Range
- null
- Freshness
- Last updated 2026-04-03 17:49:32
- Geography
- null