Primer Sequences for SUC2 and MYC2 Gene Studies in Oilseed Rape

Primer sequences used in a study investigating the role of the sucrose transporter BnaA07.SUC2 and its regulator BnaA05.MYC2 in clubroot disease susceptibility in oilseed rape (Brassica napus). The dataset, authored by Jiaqi Liu, was last updated on figshare in May 2026. It is a small, 12.3 KB XLSX file containing the specific DNA primers for gene knockout, overexpression, and expression analysis.

Use Cases

• Replicate gene knockout or overexpression experiments based on the provided CRISPR/Cas9 and overexpression primer sequences.
• Design follow-up qPCR assays to measure gene expression of BnaA07.SUC2 or BnaA05.MYC2 based on the primer sequences.
• Conduct phylogenetic or sequence analysis of sucrose transporter genes in related plant species using the primer reference points.

Strengths

• Specific primer sequences are provided for replicating key molecular biology experiments described in the associated study.
• Dataset is openly available under a CC-BY-4.0 license, permitting reuse with attribution.
• File is small (12.3 KB) and in a widely accessible XLSX format.

Limitations

• Row count and specific column names are unknown, requiring inspection after download to understand the data structure.
• The dataset's utility is highly specific to the molecular biology techniques and genes (BnaA07.SUC2, BnaA05.MYC2) described in the accompanying paper.

Provenance

Source

figshare, authored by Jiaqi Liu.

Collection Method

Primers were likely designed and used for molecular cloning, CRISPR/Cas9 editing, and gene expression analysis in the referenced study.

Freshness

Last updated 2026-05-05 17:39:16