Analytes from Canine Cancer Cell Lines for Macrophage Polarization Correlation

Rachel V. Brady's dataset contains analytes for further analysis from a study of tumor-associated macrophages in canine cancers. The data likely includes cytokine and chemokine measurements from supernatants after exposing monocyte-derived macrophages to tumor-conditioned media from 25 canine cancer cell lines. The dataset was last updated on 2026-04 24 and is shared under a CC-BY-4.0 license.

Use Cases

• Correlating cancer cell gene expression with macrophage polarization capacity based on modified z-score rankings.
• Identifying exosome-mediated polarization mechanisms based on correlations with MVB12A and VEGF stimulation.
• Investigating histiocytic sarcoma-specific drivers of macrophage TNF-α secretion based on CCL3 correlations.
• Analyzing pathways for immune suppression, EMT, and extracellular matrix remodeling based on gene set enrichment results.

Strengths

• Data is derived from a controlled experimental setup using 25 canine cancer cell lines and macrophages from three donors.
• Analysis includes RNA-sequencing correlation and differential expression, suggesting a multi-omics approach.
• Specific mechanistic insights, such as exosome fractionation confirming VEGF induction, are described.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is very small at 5.5 KB, indicating limited scope, likely summary results rather than raw data.

Provenance

Source

Rachel V. Brady via figshare

Collection Method

Experimental: Tumor-conditioned media from cell lines applied to monocyte-derived macrophages, with supernatant analysis and RNA-sequencing correlation.

Time Range

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Freshness

Last updated 2026-04-24 17:32:36; freshness should be verified.

Geography

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