Mycobacterium tuberculosis RB-TnSeq Library Screens for 95 Conditions

A pooled random barcode transposon-site sequencing (RB-TnSeq) library was generated for Mycobacterium tuberculosis. The dataset results from 95 genetic screens on carbon sources, nitrogen sources, stressors, and antibiotics, identifying 187 novel phenotypes across 37 genes in the pe/ppe family. The data was authored by Kayla M. Dinshaw and last updated on 2026-04-15.

Use Cases

• Identify genes essential for growth on specific carbon or nitrogen sources based on the 95 condition screens.
• Study antibiotic resistance mechanisms based on screens that identified a mutant resistant to pretomanid.
• Investigate the function of the pe and ppe gene family based on the 187 novel phenotypes uncovered.
• Model metabolic pathways, such as lactate utilization, based on genetic screen results.
• Prioritize drug targets by analyzing gene fitness scores under various stressors.

Strengths

• Library enables high-throughput genetic screening without standard TnSeq laborious protocols.
• Screens cover 95 distinct conditions including antibiotics, stressors, and nutrients.
• Results include 187 novel phenotypes identified for the pe/ppe gene family.
• Data is shared under a permissive CC-BY-4.0 license.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is very small at 16.5 KB, indicating limited scope.

Provenance

Source

figshare

Collection Method

Generated from a pooled random barcode transposon-site sequencing (RB-TnSeq) library in Mycobacterium tuberculosis.

Freshness

Last updated 2026-04-15 18:00:15; freshness should be verified.