Antibody List and Subpanel Allocations for Human Lymph Node Spatial Proteomics

A 78-marker antibody panel used to classify 77 cell types in 19 normal human lymph nodes. The dataset, created by Maddalena M. Bolognesi and last updated in April 2026, supports spatial proteomic analysis via the MILAN staining method and BRAQUE bioinformatic pipeline.

Use Cases

• Training cell type classification models based on the 78-marker antibody panel.
• Analyzing spatial cell-cell interactions based on the pairwise neighborhood analysis mentioned.
• Mapping novel B-cell and dendritic cell niches within lymph node architecture.
• Validating hyperplexed cyclic staining methods like MILAN for tissue proteomics.

Strengths

• Includes a detailed 78-marker antibody panel for high-dimensional proteomic interrogation.
• Based on analysis of 19 human lymph nodes, providing a multi-sample foundation.
• Classified 77 distinct cell types, including T, B, innate immune, and stromal cells.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is very small (28.0 KB), indicating limited scope, likely containing only the panel list and allocations.

Provenance

Source

figshare

Collection Method

Generated using the MILAN hyperplexed cyclic staining method and BRAQUE analytical pipeline.

Time Range

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Freshness

Last updated 2026-04-21 17:54:10; freshness should be verified.

Geography

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