Association of 5' UTR Barcodes with Cis-Regulatory Sequences

Kyle Leix published a dataset on figshare in March 2026 detailing associations between 5' UTR barcode sequences and cis-regulatory test sequences. The data lists read counts for each barcode-CRS combination and the share of reads for a barcode matching a specific CRS, filtered for combinations with at least 10 reads and barcodes with 99% read association to the same CRS. It is available as a 6.5 MB XLSX file under a CC-BY-4.0 license.

Use Cases

• Training models to predict cis-regulatory element activity based on 5' UTR barcode associations.
• Analyzing the strength and specificity of regulatory sequences mentioned in the description.
• Investigating the impact of genetic variants on gene expression using barcode-CRS pairing data.

Strengths

• Data is filtered for quality, removing combinations with less than 10 reads.
• Includes a stringent filter for barcodes, requiring 99% of reads to be associated with the same CRS.
• Published under a permissive CC-BY-4.0 license, facilitating reuse.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare

Collection Method

Likely generated from a molecular biology assay interrogating candidate cis-regulatory elements.

Time Range

null

Freshness

Last updated 2026-03-17 17:32:06; freshness should be verified.

Geography

null