Pseudomonas Aeruginosa Detection Data for a Novel RPA-Cas12b Assay

Haotian Lin published this dataset on figshare in March 2026. It contains data related to a novel rapid detection platform for the opportunistic pathogen Pseudomonas aeruginosa. The dataset likely includes results from sensitivity, specificity, and validation assays described in the study.

Use Cases

• Benchmarking the sensitivity of molecular detection methods based on the reported 10-copy DNA threshold.
• Evaluating assay specificity for Pseudomonas aeruginosa against related species based on the described cross-reactivity tests.
• Validating rapid diagnostic platforms for water sample analysis based on the 20-sample validation mentioned.
• Optimizing reaction conditions for recombinase polymerase amplification (RPA) and CRISPR-Cas12b systems.

Strengths

• Specific detection limits are provided: 10 copies of DNA and 50 CFU per reaction for the fluorescence system.
• High specificity was confirmed with no cross-reactivity against Pseudomonas fluorescens, Pseudomonas putida, or six common foodborne pathogens.
• Method validation showed 100% concordance with a national standard culture method for 20 water samples.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is very small at 5.5 KB, indicating limited scope.

Provenance

Source

figshare, author Haotian Lin.

Collection Method

Data likely generated from laboratory experiments optimizing and validating a novel RPA-CRISPR/Cas12b detection platform.

Time Range

Temporal coverage of the experimental data is not specified.

Freshness

Last updated 2026-03-18 17:33:31; freshness should be verified.

Geography

Spatial coverage is not specified.