Mycobacterium Phage Lysis Gene Deletion Phenotypes

Experimental data details the lysis phenotypes of two *Mycobacterium smegmatis* phages following targeted gene deletions. The dataset includes results from plaque size assays, lysis timing in liquid culture, and burst size measurements for mutants. Richard S. Pollenz published this work in April 2026.

Use Cases

• Analyze correlations between lysF1a and lysF1b gene deletions and resulting plaque size phenotypes.
• Model lysis timing in liquid culture as a function of specific holin-like protein deletions.
• Investigate the impact of lipomannan/lipoarabinomannan-deficient bacterial strains on phage plaque size.
• Compare burst size reductions (~65%) in lysis recovery mutants with specific point mutations in the lysF1a gene.

Strengths

• Data is published under a permissive CC-BY-4.0 license for reuse.
• Findings are based on controlled experimental deletions of specific genes (lysF1a, lysF1b).

Limitations

• The dataset is very small at 9.5 KB, indicating limited scope and sample size.
• Specific row counts, column names, and raw measurement values are not provided in the description.

Provenance

Source

figshare, authored by Richard S. Pollenz.

Collection Method

Experimental laboratory research involving phage genetics, phenotypic assays, and genome sequencing.

Time Range

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Freshness

Last updated in April 2026.

Geography

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