Mtb RB-TnSeq Library: 95 Genetic Screens on Carbon Sources and Antibiotics

A pooled random barcode transposon-site sequencing (RB-TnSeq) library for Mycobacterium tuberculosis (Mtb). The dataset includes results from 95 genetic screens on an array of carbon sources, nitrogen sources, stressors, and antibiotics, generated by Kayla M. Dinshaw. It was last updated on 2026-04-15.

Use Cases

• Predicting gene essentiality under specific nutrient conditions based on the 95 RB-TnSeq screens.
• Investigating the function of the pe and ppe gene family based on 187 novel phenotypes uncovered for 37 genes.
• Modeling metabolic pathways, such as lactate utilization, based on the proposed role of the ESX-5 secretion system and a candidate D-lactate dehydrogenase.
• Identifying genetic determinants of antibiotic resistance based on the novel mutant conferring resistance to pretomanid.

Strengths

• Dataset is derived from 95 distinct RB-TnSeq genetic screens.
• The library design includes unique 20-nucleotide barcodes for high-throughput screening.
• Results include 187 novel phenotypes identified across 37 genes in the pe/ppe family.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is small (14.6 KB), indicating a limited scope of raw or summary data.

Provenance

Source

figshare

Collection Method

Generated via pooled random barcode transposon-site sequencing (RB-TnSeq) library construction and screening.

Freshness

Last updated 2026-04-15 18:00:13; freshness should be verified.