Circular RNAs in Brassica Napus Phloem Sap with 1,734 Identified circRNAs

Kim Lara Lühmann's dataset characterizes circular RNA content in the phloem sap of the crop plant Brassica napus. The analysis identified 1,734 distinct circRNAs, with ten validated by PCR and Sanger sequencing, and investigated full-length sequences and potential miRNA interactions. The data was last updated on 2026-04-29.

Use Cases

• Identify potential long-distance signaling circRNA candidates based on the 1,734 distinct circRNAs discovered.
• Analyze circRNA isoform variation and intron retention based on full-length sequences from phloem and leaf samples.
• Investigate circRNA-miRNA interactions based on identified target sites for phloem-mobile miRNAs like miR156, miR169, and miR395.
• Study RNA-binding protein interactions with circRNAs based on the measured binding affinity of BnGRP7 to circBnaANL2(7,8).

Strengths

• Contains 1,734 distinct circRNAs identified from phloem sap.
• Includes validation data for ten circRNAs via PCR and Sanger sequencing of back-splicing junctions.
• Provides full-length sequences for 14 circRNAs from phloem and leaf tissues via Nanopore sequencing.
• Dataset is openly available under a CC-BY-4.0 license.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is small in scale at 708.2 KB, indicating limited scope.

Provenance

Source

figshare

Collection Method

Data generated via Illumina sequencing of rRNA-depleted, circRNA-enriched, and sRNA libraries, with validation by PCR, Sanger sequencing, and Nanopore sequencing.

Freshness

Last updated 2026-04-29 17:39:02; freshness should be verified.