Cytomix Buffer Composition and Electroporation Performance Scores

A 5.5 KB Excel file containing the composition of a modified Opti-MEM I based electroporation buffer for Toxoplasma gondii and a novel scoring method (etScore) for quantifying electroporation performance. The dataset was created by Pratik Narain Srivastava and last updated in April 2026.

Use Cases

• Compare the performance of the modified buffer composition against traditional cytomix using the provided etScore metrics.
• Analyze the relationship between specific buffer components and the combined transgene expression and cell viability scores.
• Benchmark new electroporation experiments against the established etScore values for reproducibility assessment.
• Use the buffer composition data to replicate the simplified protocol for Toxoplasma gondii transfection.

Strengths

• Dataset is openly licensed under CC-BY-4.0.
• Includes a novel, reproducible scoring method (etScore) combining two key performance metrics.

Limitations

• Extremely small dataset at 5.5 KB, indicating limited scope and likely few rows of data.
• Data format is a legacy XLS file, which may require conversion for modern analysis tools.
• No sample data or column names are provided, making initial assessment difficult.

Provenance

Source

figshare, authored by Pratik Narain Srivastava.

Collection Method

Derived from experimental work optimizing electroporation protocols for Toxoplasma gondii.

Time Range

null

Freshness

Last updated in April 2026.

Geography

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