Leishmania Major p1/s1 Nuclease Proteomics and Activity Data

Proteomic and enzymatic activity data for the p1/s1 nuclease cluster in Leishmania major parasites. The dataset was generated by Stella M. Schmelzle using quantitative proteomics, immunological assays, and genome editing methods. It was last updated on 2026-05-21.

Use Cases

• Identify compensatory protein expression changes in knockout strains based on proteomic enrichment data.
• Analyze ecto-enzymatic activity levels in parasites and culture supernatants under different treatments.
• Model the role of 3'-nucleotidases in immune modulation based on cytokine secretion and lymphocyte proliferation assays.
• Investigate nuclease-mediated degradation of neutrophil extracellular traps as a survival mechanism.

Strengths

• Data is based on quantitative proteomics, a high-resolution measurement technique.
• Includes results from viable null mutants generated via a diCre-based inducible knockout system.
• File size is 4.3 MB, indicating a focused and manageable dataset.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

Stella M. Schmelzle

Collection Method

Generated via quantitative proteomics, immunological assays, and genome editing (diCre-based inducible knockout).

Freshness

Last updated 2026-05-21 07:37:45; freshness should be verified.