Leishmania Major p1/s1 Nuclease Proteomics and Activity Data

Stella M. Schmelzle's dataset characterizes the p1/s1 cluster of 3'-nucleotidases/nucleases in Leishmania major parasites. It includes proteomic data from quantitative proteomics, ecto-enzymatic activity measurements, and results from a diCre-based inducible knockout system. The dataset was last updated on 2026-05-21 and is available as a 4.5 MB XLSX file under a CC-BY-4.0 license.

Use Cases

• Analyzing differential protein expression in Leishmania major under drug treatment (miltefosine or staurosporine) based on proteomic data.
• Studying the link between ecto-nuclease activity and immune evasion mechanisms like neutrophil extracellular trap degradation.
• Investigating compensatory protein enrichment in knockout strains deficient for the p1/s1 nuclease cluster.
• Modeling the role of extracellular adenosine generation in suppressing macrophage cytokine secretion and lymphocyte proliferation.

Strengths

• Dataset is based on an integrative approach combining proteomics, immunological assays, and genome editing methods.
• Includes data on enzyme activity from both parasites and culture supernatants, as described.
• Last update date is 2026-05-21, indicating recent curation.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The 4.5 MB size suggests a relatively small-scale dataset.

Provenance

Source

Stella M. Schmelzle, via figshare.

Collection Method

Data generated using quantitative proteomics, genome editing (diCre-based knockout), and enzymatic activity assays on Leishmania major parasites.

Freshness

Last updated 2026-05-21 07:37:44