DESeq2 Results for Drosophila Sleep Onset from Peripheral Tissue Manipulation

DESeq2 differential gene expression analysis results from a study on sleep regulation in Drosophila melanogaster. The data, 2.3 MB in CSV format, was published by Josué A. Rodríguez-Cordero on figshare in April 2026. It captures transcriptional changes in fly heads following genetic manipulation of the RNA-binding protein Pumilio in intestinal stem cells and endocrine tissue.

Use Cases

• Identify differentially expressed genes (log2FoldChange, pvalue) associated with accelerated sleep onset after Pumilio knockdown in intestinal stem cells.
• Analyze gene ontology enrichment among upregulated stress response genes (padj) from head tissue transcriptomes.
• Compare expression profiles (baseMean) between experimental conditions targeting the corpus allatum versus intestinal stem cells.
• Validate transcriptional signatures of peripheral-to-central signaling by correlating gene expression changes with behavioral sleep metrics.

Strengths

• Data is derived from a controlled genetic study with conditional tissue targeting, providing specific mechanistic insights.
• Published under a permissive CC-BY-4.0 license allowing reuse and redistribution.
• Results are from a DESeq2 analysis, a standard and reproducible method for RNA-seq differential expression.

Limitations

• The 2.3 MB file size suggests a limited number of genes or samples, potentially restricting broad statistical power.
• Dataset focuses on a single model organism (Drosophila), limiting direct translatability to mammalian systems.
• Specific sample size (number of biological replicates) and raw read counts are not provided, complicating depth assessment.

Provenance

Source

figshare, authored by Josué A. Rodríguez-Cordero.

Collection Method

RNA sequencing and subsequent differential expression analysis using DESeq2 on Drosophila melanogaster head tissue following genetic perturbations.

Time Range

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Freshness

Last updated in April 2026, indicating recent analysis and publication.

Geography

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