A 23.0 KB Excel file containing designs for intron-targeting single-guide RNAs (sgRNAs) aimed at selectively ablating the endogenous HNRNPK gene. The dataset was authored by Stefano Sellitto and last updated on April 20, 2026. It is shared under a CC-BY-4.0 license on the figshare platform.
Use Cases
- Designing CRISPR-Cas9 gene knockout experiments based on intron-targeting sgRNA sequences.
- Comparing sgRNA efficacy and specificity for the HNRNPK gene based on the provided designs.
- Developing protocols for selective gene ablation based on the described intron-targeting approach.
Strengths
- Dataset is openly licensed under CC-BY-4.0, permitting reuse with attribution.
- File size is 23.0 KB, indicating a focused and manageable scope for the designs.
Limitations
- Description metadata is limited; actual data quality requires manual inspection after download.
- Column-level documentation is absent; field semantics must be inferred after download.
- Row count is unknown, which may limit suitability assessment.
Provenance
- Source
- figshare
- Collection Method
- Likely contains computational designs or experimental plans for sgRNAs.
- Time Range
- Publication date is April 20, 2026.
- Freshness
- Last updated 2026-04-20 17:40:21; freshness should be verified.
- Geography
- null