Plasmids for Pneumocystis DHFR Deep Mutational Scanning Study

Francois D. Rouleau published details on plasmids used in a study of Pneumocystis jirovecii dihydrofolate reductase (PjDHFR) on 2026-05-27. The dataset, shared under a CC-BY-4.0 license, is a 10.7 KB XLSX file. It supports research on antifungal drug resistance by detailing genetic constructs for deep mutational scanning experiments in yeast.

Use Cases

• Replicating deep mutational scanning experiments based on the described plasmid constructs.
• Training interpretable machine learning models for drug resistance prediction based on functional and biophysical features mentioned in the description.
• Analyzing the functional-resistance trade-off in PjDHFR mutations as described in the associated study.
• Modeling the effects of mutations on resistance to trimethoprim using the described experimental framework.

Strengths

• Dataset is shared under a permissive CC-BY-4.0 license, facilitating reuse.
• Specific file size of 10.7 KB indicates a focused, non-redundant dataset.
• Description provides detailed scientific context linking the plasmids to a published study on deep mutational scanning.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare

Collection Method

Likely generated as part of a laboratory study involving plasmid construction and deep mutational scanning.

Freshness

Last updated 2026-05-27 17:44:21; freshness should be verified.