Diagnostic Performance Metrics for Tomato Leaf Curl Virus Detection

A 5.5 KB Excel file contains performance metrics for a novel diagnostic assay. The probe-enhanced LAMP assay developed by Abigarl Ndudzo detected Kenyan tomato leaf curl virus in 2.7 minutes under optimal conditions and was 10^6 times more sensitive than conventional PCR. The data, last updated in May 2026, supports the application of this assay for phytopathological testing in resource-poor areas.

Use Cases

• Benchmarking diagnostic sensitivity based on the reported analytical sensitivity of 0.001 fg/μL
• Comparing assay performance times based on the reported time-to-positive of ~4.8 minutes
• Evaluating field applicability based on the detection of the virus in 50% of asymptomatic samples
• Assessing specificity for quarantine inspection based on the reported lack of cross-reaction with other viruses

Strengths

• Includes specific performance metrics such as a 2.7-minute detection time and 10^6-fold sensitivity increase over PCR
• Data is openly licensed under CC-BY-4.0 for reuse
• Assay validation details are provided, including testing on asymptomatic samples

Limitations

• Row count is unknown, which may limit suitability assessment
• Column-level documentation is absent; field semantics must be inferred after download
• The dataset is very small at 5.5 KB, indicating limited scope

Provenance

Source

figshare, authored by Abigarl Ndudzo

Collection Method

Metrics derived from laboratory experiments optimizing a LAMP assay and a modified DNA extraction method

Time Range

null

Freshness

Last updated 2026-05-22 17:32:26; freshness should be verified

Geography

Focus on Kenyan tomato leaf curl virus isolates; specific sample geography is not detailed