Diagnostic Performance Metrics for Kenyan Tomato Leaf Curl Virus Detection

A Kenyan study developed a probe-enhanced loop-mediated isothermal amplification (LAMP) assay for detecting tomato leaf curl virus isolates in host crops. The dataset, authored by Abigarl Ndudzo and last updated in May 2026, contains performance metrics for the assay, including sensitivity and time-to-positive results. The assay was optimized for use in resource-poor settings and tested on symptomatic and asymptomatic tomato and chili pepper samples.

Use Cases

• Benchmarking diagnostic sensitivity based on the reported 0.001 fg/μL detection limit.
• Comparing assay speed based on the 2.7-minute detection time under optimal conditions.
• Evaluating field applicability for asymptomatic detection based on the 50% success rate in asymptomatic samples.
• Assessing specificity for quarantine inspection based on the described lack of cross-reaction with other viruses.

Strengths

• Assay sensitivity reached 0.001 fg/μL of total DNA.
• The optimized LAMP assay was reported to be 10^6 times more sensitive than conventional PCR.
• The modified extraction buffer yielded a significantly shorter time-to-positive (~4.8 minutes; p < 0.0001).

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.

Provenance

Source

figshare, authored by Abigarl Ndudzo.

Collection Method

Data likely derived from laboratory experiments evaluating a diagnostic assay.

Time Range

null

Freshness

Last updated 2026-05-22 17:32:26; freshness should be verified.

Geography

Kenya (implied by the focus on Kenyan tomato leaf curl virus isolates).