Enzymatic Activity Data for Bacteriophage-Derived Chimeric Protein Variants

A 5.5 KB Excel file containing experimental results for chimeric protein variants created by fusing domains from KP32gp38 and K11gp0043 bacteriophage proteins. The dataset, authored by Agnieszka Latka and shared via figshare, records protein expression levels and enzymatic activity halos against specific bacterial lawns. It was last updated in April 2026.

Use Cases

• Correlate protein domain origin (KP32 or K11) with enzymatic activity results against bacterial lawns like K31, K21, and KL163.
• Analyze the impact of structural components (linker, CBM, lectin-like domain) on protein expression and halo formation.
• Validate or compare AlphaFold 3 predicted 3D structures against experimental activity data for chimeric variants.

Strengths

• Data is explicitly linked to a published figure (Fig 2) in a PLOS Pathogens article, providing academic context.
• Includes specific experimental condition notes, such as 'NO' for expression without activity and 'nd' for no expression.

Limitations

• The dataset is very small at 5.5 KB, indicating a limited experimental scope with few variants or measurements.
• Contains an acknowledged data inconsistency marked with an asterisk for results on the KL163 bacterial lawn.

Provenance

Source

Agnieszka Latka, associated with research published in PLOS Pathogens.

Collection Method

Experimental data from protein expression (SDS-PAGE) and enzymatic activity assays on bacterial lawns.

Freshness

Last updated in April 2026.