Essential Genes from TnSeq Data: Mycobacterium Tuberculosis RB-TnSeq Library

95 RB-TnSeq screens on Mycobacterium tuberculosis across carbon sources, nitrogen sources, stressors, and antibiotics. The dataset, generated by Kayla M. Dinshaw, contains 187 novel phenotypes identified for the elusive pe and ppe gene family. It was last updated on 2026-04-15.

Use Cases

• Identify essential genes for growth under specific nutrient conditions based on the 95 genetic screens.
• Investigate the function of the pe and ppe gene family based on the 187 novel phenotypes.
• Study metabolic pathways like lactate and propionate utilization based on the proposed mechanisms.
• Analyze genetic determinants of antibiotic resistance based on the pretomanid resistance mutant.

Strengths

• Contains results from 95 distinct genetic screens.
• Identifies 187 novel phenotypes for the pe/ppe gene family.
• Uses a high-throughput RB-TnSeq method for efficient screening.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data may reflect experimental bias inherent to the specific RB-TnSeq library.

Provenance

Source

Kayla M. Dinshaw via figshare.

Collection Method

Generated from a pooled random barcode transposon-site sequencing (RB-TnSeq) library in Mycobacterium tuberculosis.

Freshness

Last updated 2026-04-15 18:00:11; freshness should be verified.