Experimental Design Levels for Sulfated Polysaccharide Extraction and Bioactivity

Zeynab Falihzadeh published a dataset on figshare in 2026 detailing the experimental design for optimizing sulfated polysaccharide extraction from red algae Gracilaria corticata. The dataset likely contains parameters and results from extraction optimization, chemical characterization, and bioactivity assays. It is a small dataset of 5.5 KB, stored in an XLS file.

Use Cases

• Optimizing ultrasound-assisted extraction parameters based on documented time, power, and solvent ratios.
• Modeling the relationship between polysaccharide chemical composition and antioxidant activity based on DPPH/ABTS assay results.
• Analyzing dose-response cytotoxicity against HT-29 cancer cells based on reported IC50 and selectivity index values.
• Studying apoptosis induction pathways based on gene expression markers like Bax, P53, Caspase 3, and BCL2.

Strengths

• Specific extraction yield of 34.8% sulfated polysaccharides is reported.
• Detailed chemical composition is provided, including 84.4% carbohydrates and 8.7% sulfate.
• Bioactivity results include specific IC50 (171 μg/mL) and radical scavenging percentages (81.92% DPPH, 86.34% ABTS).

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset's 5.5 KB size suggests a very limited scope, likely containing summary or design-level data rather than raw observations.

Provenance

Source

Zeynab Falihzadeh via figshare

Collection Method

Data likely generated from laboratory experiments involving ultrasound-assisted extraction, biochemical assays, and cell culture studies.

Freshness

Last updated 2026-05-18 17:27:43; freshness should be verified.