C. elegans Ethanol Exposure and Mutant Response Data

Experimental data from a study on the effects of 24-hour ethanol exposure, withdrawal, and withdrawal relief on Caenorhabditis elegans. The research employed a modified kinetic chemotaxis assay, lifespan analysis, mobility tests, gene expression analysis, and imaging assays to evaluate sensorimotor performance and molecular alterations. Results include differential responses in wild-type, dopamine receptor mutant, and serotonin biosynthesis null mutant worms.

Use Cases

• Analyze differential responses to ethanol exposure between wild-type, dopamine receptor mutant, and serotonin biosynthesis null mutant worms in kinetic chemotaxis assays.
• Correlate gene expression analysis data from stress response genes with behavioral outcomes from the diacetyl race paradigm.
• Model drug tolerance using the 3-step experimental framework based on ethanol exposure, withdrawal, and withdrawal relief phases.
• Investigate the impact of 24-hour ethanol exposure on lifespan data across different genetic strains of C. elegans.
• Assess alterations in vesicle exocytosis in dopaminergic and serotonergic neurons from imaging assays following ethanol treatment.

Strengths

• Data originates from a controlled laboratory study employing multiple assay types, including kinetic chemotaxis, lifespan, mobility, gene expression, and imaging.
• Includes comparative analysis across three distinct genetic models: wild-type, a dopamine receptor mutant, and a serotonin biosynthesis null mutant.
• The experimental model is explicitly designed as a 3-step framework for studying drug tolerance, based on the established diacetyl race behavioral paradigm.

Limitations

• The dataset is very small at 5.5 KB, indicating limited scope and likely a summary or aggregated results rather than raw experimental observations.
• Specific sample sizes, row counts, and detailed columnar data are unavailable, limiting direct analytical utility.
• Data is confined to the model organism C. elegans, and direct translation to human alcohol use disorder requires significant additional research.

Provenance

Source

figshare, authored by Teresa Rubio-Tomás.

Collection Method

Data generated from laboratory experiments on C. elegans, including a modified kinetic chemotaxis assay, lifespan tracking, mobility tests, gene expression analysis, and imaging assays.

Time Range

Covers a 24-hour ethanol exposure period and subsequent withdrawal phases.

Freshness

Last updated March 23, 2026.