E. coli Lipidomics Dataset for Tox-Act1 Wild-Type and Mutant Strains

A full dataset of identified lipids from E. coli bacteria expressing either the wild-type Tox-Act1 protein or its C151A mutant variant. The data was generated using LC-MS/MS methods and includes phospholipids and lysophospholipids analyzed in negative mode. Author Gianlucca G. Nicastro published the dataset on figshare under a CC-BY-4.0 license, with a last update in March 2026.

Use Cases

• Identify statistically altered lipids between bacterial strains based on the comparative analysis mentioned.
• Validate LC-MS/MS methods for phospholipid and lysophospholipid detection using the described MRM transitions.
• Study the impact of specific protein mutations (C151A) on bacterial lipid profiles.
• Use the internal standard information for calibrating negative-mode lipidomics analyses.

Strengths

• Dataset is openly licensed under CC-BY-4.0.
• Specific analytical methods (LC-MS/MS, negative mode) are described.
• Focus on a well-defined biological comparison (Tox-Act1WT vs. Tox-Act1C151A).

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is very small at 33.4 KB, indicating limited scope.

Provenance

Source

figshare

Collection Method

Likely generated via laboratory LC-MS/MS lipidomics analysis.

Time Range

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Freshness

Last updated 2026-03-17 17:30:01; freshness should be verified.

Geography

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