Gene Primer Sequences for Apoptosis Analysis in qPCR Experiments

Gene primer sequences used in quantitative PCR (qPCR) analysis within a study of sulfated polysaccharides from red algae. The dataset supports research into the antioxidant and anticancer properties of Gracilaria corticata extracts. It was authored by Zeynab Falihzadeh and last updated on May 18, 2026.

Use Cases

• Designing qPCR assays for apoptosis-related genes based on the provided primer sequences.
• Validating gene expression changes (e.g., Bax, P53, Caspase 3, BCL2) in cytotoxicity studies.
• Replicating or extending the analysis of sulfated polysaccharide effects on HT-29 colon cancer cells.

Strengths

• Primer sequences are directly linked to a published study with specific results, including an IC₅₀ of 171 μg/mL and a selectivity index of 3.48.
• Dataset is openly licensed under CC-BY-4.0, permitting reuse with attribution.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is very small (5.5 KB), indicating a limited scope focused on primer sequences.

Provenance

Source

figshare

Collection Method

Likely generated as part of a laboratory study involving ultrasound-assisted extraction, biochemical assays, and cell culture experiments.

Freshness

Last updated 2026-05-18 17:27:44; freshness should be verified.