Gene Expression in ATG5 and ATG7-Silenced Endothelial Cells via PCR Array

Aman Singh's dataset contains gene expression data for endothelial cells with silenced ATG5 versus control cells, with additional status information for ATG7-silenced cells. The data was collected 24 hours post-transfection using a TGF-ß/BMP signaling pathway PCR array. The dataset is shared under a CC-BY-4.0 license on figshare.

Use Cases

• Identify genes up-regulated in response to ATG5 silencing based on the described experimental comparison.
• Analyze the overlap or divergence in gene expression between ATG5 and ATG7 silencing within the TGF-ß/BMP pathway.
• Validate PCR array findings for specific signaling pathway components mentioned in the description.
• Investigate the role of autophagy-related genes in endothelial cell signaling 24 hours post-transfection.

Strengths

• Data is focused on a specific biological pathway (TGF-ß/BMP) and experimental condition (24 hours post-transfection).
• Dataset is openly shared under a permissive CC-BY-4.0 license.
• File format is XLS, which is widely accessible for initial inspection.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for large-scale analysis.
• The dataset is very small at 9.5 KB, indicating limited scope.

Provenance

Source

Aman Singh

Collection Method

Data collected 24 hours post-transfection using a TGF-ß/BMP signaling pathway PCR array.

Time Range

Data collection timepoint is specified, but overall study period is unknown.

Freshness

Last updated 2026-04-30 17:34:28; freshness should be verified.

Geography

Spatial coverage is unknown.