Mtb RB-TnSeq Library: Genetic Fitness Across 95 Carbon, Nitrogen, and Stress Screens

Kayla M. Dinshaw's dataset contains pooled random barcode transposon-site sequencing (RB-TnSeq) library data for Mycobacterium tuberculosis (Mtb). It includes results from 95 genetic screens on carbon sources, nitrogen sources, stressors, and antibiotics, identifying 187 novel phenotypes for the pe/ppe gene family. The data was last updated on 2026-04-15 and is shared under a CC-BY-4.0 license.

Use Cases

• Identify genes essential for growth on specific carbon or nitrogen sources based on the 95 RB-TnSeq screens.
• Investigate the function of the elusive pe and ppe gene family based on the 187 novel phenotypes uncovered.
• Study metabolic pathways, such as lactate utilization, based on the proposed role of the ESX-5 secretion system and PPE3.
• Analyze genetic determinants of antibiotic resistance based on the novel mutant identified for pretomanid.
• Generate hypotheses about uncharacterized gene function based on the cofitness patterns across diverse conditions.

Strengths

• Includes results from 95 distinct genetic screens, covering a wide array of conditions.
• Identifies 187 novel phenotypes for the poorly understood pe/ppe gene family.
• Uses a high-throughput RB-TnSeq method that facilitates rapid genetic screening.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is small (24.9 KB), indicating limited scope, likely containing summary or processed results rather than raw sequencing data.

Provenance

Source

Kayla M. Dinshaw via figshare.

Collection Method

Generated from a pooled random barcode transposon-site sequencing (RB-TnSeq) library in Mycobacterium tuberculosis.

Freshness

Last updated 2026-04-15 18:00:18; freshness should be verified.