Guide and Primer Sequences for Mouse Placenta Replication Stress Study

Mumingjiang Munisha published a 9.7 KB dataset on figshare in April 2026. The dataset contains guide and primer sequences used in a study investigating how replication stress from MCM2–7 helicase mutations disrupts placentation and reduces embryo viability in mice. The work examines impacts on trophoblast stem cells and the placental junctional zone.

Use Cases

• Designing CRISPR-Cas9 gene editing experiments based on the provided guide sequences
• Performing PCR amplification and genotyping based on the primer sequences
• Replicating the study's molecular biology assays on MCM2–7 and FANCM deficiencies
• Analyzing genetic factors in trophoblast stem cell maintenance and placental zone development

Strengths

• File size is precisely 9.7 KB, indicating a focused and manageable dataset.
• Dataset is openly licensed under CC-BY-4.0, permitting reuse with attribution.
• Last update timestamp is provided (2026-04-13 17:37:57), suggesting recent activity.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare, author Mumingjiang Munisha.

Collection Method

Likely generated as part of a molecular biology study on mouse models.

Time Range

Publication date suggests work circa 2026.

Freshness

Last updated 2026-04-13 17:37:57; freshness should be verified.

Geography

Geographic coverage is not specified in the provided metadata.