Harmonized Lymph Node Cell Subsets: 77 Types from 19 Human Samples

19 human lymph nodes free of pathology were classified in situ using a 78-marker antibody panel and the MILAN cyclic staining method. A total of 77 cell types were identified, encompassing T, B, innate immune, and stromal cells. The dataset was created by Maddalena M. Bolognesi and last updated on 2026-04-21.

Use Cases

• Classifying T-cell subsets based on expression profiles of TCF7 and co-inhibitory receptors mentioned in the description
• Mapping novel B-cell types defined by CD5 and TCF7 expression within lymph node spatial niches
• Analyzing statistically controlled pairwise neighborhood interactions between immune and stromal cells
• Investigating the spatial allocation of type 2 conventional dendritic cells in paracortical aggregates

Strengths

• Data is derived from 19 human lymph node samples, providing a multi-sample foundation
• Classification includes 77 distinct cell types, offering high granularity
• Generated using a 78-marker antibody panel, suggesting a high-dimensional proteomic profile

Limitations

• Row count is unknown, which may limit suitability assessment
• Column-level documentation is absent; field semantics must be inferred after download
• The dataset is very small at 10.8 KB, indicating limited scope

Provenance

Source

figshare

Collection Method

Hyperplexed cyclic staining (MILAN) and analytical bioinformatic pipeline (BRAQUE) applied to human tissue samples.

Freshness

Last updated 2026-04-21 17:54:26; freshness should be verified